DNA sequencing facilities

Facilities are available in the Mishler Lab itself for pre-PCR DNA work. Extractions are prepared here using two fume hoods, - 20°C freezer, refrigerator, Fisher waterbath, Dupont Sorvall and a MC12V microcentrifuge. Once PCR reactions are set up, we move to the Molecular Phylogenetics Laboratory (where Mishler is one of four co-PIs) for amplification and sequencing.

The Molecular Phylogenetics Laboratory (MPL) is located in a two-room suite (rooms 1123 and 1125) on the first floor of the Valley Life Sciences Building near the Herbarium, and is a joint venture of the University and Jepson Herbaria with the Museum of Paleontology. The MPL is equipped for all major molecular systematic techniques. That facility includes 2 MJ Research PTC-200 Thermo Cycler,1 Perkin Elmer Gene Amp PCR Systems, Stratagene Eagle Eye Gel Imaging System, Savant Speed Vac System, Sorvall MC12V microcentrifuge, multiple VWR heatblocks, -20°C freezer, and a 4°C refrigerator.

More complete information can be found at MPL website.