The overall objective of the GTOL project is to resolve the primary pattern
of evolutionary diversification among green plants. To do this, we will generate
a comprehensive dataset for over 50 exemplar taxa. This dataset will incorporate
characters from morphology, ultrastructure, and both organellar genome and nuclear
gene sequences.
The table below serves as a public guide to our progress, indicating the status
of morphological and molecular data across all taxa. The main functions of this
public database are to indicate research that is planned or in progress by our
group AND by others. This should help to avoid unnecessary duplication and to
foster collaboration among labs.
Last Updated: 25 Feb 2005 by Paul G. Wolf.
Please email enquiries and requests for additions and changes to Paul
Wolf
  |
Shaded rows refer to taxa being studied by other research groups |
GTOL # |
Taxon name in orginal proposal |
Replacement Taxon |
Clade name |
Organelle Genome Pipeline Status |
Morphology status |
Notes |
|
Chloroplast |
Mitochondrial |
|
1 |
Nephroselmis olivacea |
|
Prasinophyte |
NC
000927 |
AF110138 |
In lit |
|
2 |
Pterosperma sp. |
|
Prasinophyte |
Tissue Prep |
Tissue Prep |
In Progress |
|
3 |
Chlorella vulgaris |
|
Trebouxiophyte |
AB001684 |
Tissue Prep |
In lit |
|
4 |
Prototheca wickerhamii |
|
Trebouxiophyte |
AJ245645 |
NC
001613 |
|
Chloroplast sequence in Genbank is partial |
5 |
Tetraselmis striata |
|
Trebouxiophyte |
OGMP |
OGMP |
In lit |
|
6 |
Volvox carteri |
|
Chlorophyte |
QC |
QC |
In lit |
Nuclear genome being sequenced |
7 |
Chlamydomonas reinhardtii |
|
Chlorophyte |
BK000554 |
U03843 |
In lit |
See web site [link] |
8 |
Oltmannsiellopsis viridis |
|
Ulvophyte B |
OGMP |
OGMP |
In lit |
|
9 |
Lewinosphaera lissoclini (CCMP 1293) |
CCMP 1293 |
Ulvophyte 2(?) |
Tissue Prep |
Tissue Prep |
In Progress |
|
10 |
Chlorocystis moorei |
|
Ulvophyte 1 |
Tissue Prep |
Tissue Prep |
In Progress |
|
11 |
Ulothrix sp. |
Pseudendoclonium akinetum |
Ulvophyte 1 |
Tissue Prep |
AY359242 |
In lit |
|
12 |
Acrochaete endozoica |
|
Ulvophyte 1 |
Tissue Prep |
Tissue Prep |
|
|
13 |
Bolbocoleon piliferum |
|
Ulvophyte 1 |
Tissue Prep |
Tissue Prep |
Complete |
|
14 |
Ulva lactuca |
Ulva californica WA1.3b |
Ulvophyte 1 |
OGMP |
OGMP |
In lit |
|
15 |
Trentepohlia sp. |
|
Ulvophyte 2 |
Tissue Prep |
Tissue Prep |
|
|
16 |
Blastophysa rhizopus |
|
Ulvophyte 2 |
RCA |
RCA |
Complete |
|
17 |
Chaetosiphon moniliformis |
|
Ulvophyte 2 |
RCA |
RCA |
Complete |
|
18 |
Wittrockiella paradoxa |
|
Ulvophyte 2 |
RCA |
RCA |
Complete |
|
19 |
Caulerpa taxifolia |
|
Ulvophyte 2 |
Tissue Prep |
Tissue Prep |
|
|
20 |
Codium decorticatum |
Codium fragile ssp. Tomentosoides |
Ulvophyte 2 |
RCA |
RCA |
In Progress |
|
21 |
Ostreobium queketii |
|
Ulvophyte 2 |
FACS |
FACS |
In Progress |
|
22 |
Acetabularia acetabulum |
|
Ulvophyte 2 |
RCA/PLUGS |
RCA/PLUGS |
In lit |
|
23 |
Bornetella sphaerica |
|
Ulvophyte 2 |
Tissue Prep |
Tissue Prep |
In lit |
|
24 |
Mesostigma viride |
|
Mesostigmatophyte |
NC
002186 |
AF353999 |
In lit |
|
25 |
Chaetosphaeridium globosum |
Coleochaete orbicularis |
Coleochaetophyte |
QC |
QC |
|
|
|
Chaetosphaeridium globosum |
|
|
AF494278 |
AF494279 |
|
|
|
Chara vulgaris |
|
Charophyte |
OGMP |
NC_005255 |
|
|
26 |
Chara braunii |
|
Charophyte |
CLONE |
CLONE |
|
|
27 |
Klebsormidium flaccidum |
|
Klebsormidiophyte |
OGMP |
OGMP |
In lit |
|
28 |
Entransia fimbriata |
|
Klebsormidiophyte |
Tissue Prep |
Tissue Prep |
In Progress |
|
29 |
Chlorokybus atmosphyticus |
|
Chlorokybophyte |
OGMP |
OGMP |
|
|
30 |
Spirogyra maxima |
|
Zygnematophyte |
Jungho Lee |
Tissue Prep |
In lit |
|
31 |
Anthoceros formosae |
|
Hornwort |
AB086179 |
Yoshinaga PLUGS |
|
KE: Organellar DNA has been obtained from the NIB extract. |
|
Anthoceros agrestis |
|
Hornwort |
|
Qiu planned |
|
|
32 |
Notothylas orbicularis |
Leiosporoceros dussii |
Hornwort |
RCA |
RCA |
|
|
33 |
Sphaerocarpos sp. |
|
Liverwort |
Tissue Prep |
Tissue Prep |
|
|
34 |
Marchantia polymorpha |
|
Liverwort |
X04465 |
NC
001660 |
|
|
35 |
Blasia pusilla |
|
Liverwort |
RCA |
RCA |
|
|
36 |
Bazzania trilobata |
|
Liverwort |
Tissue Prep |
Tissue Prep |
|
|
37 |
Haplomitrium mnioides |
|
Liverwort |
RCA |
RCA |
|
|
|
Scapania nemorosa |
|
Liverwort |
|
Qiu planned |
|
|
38 |
Sphagnum palustre |
|
Moss |
SEQ |
SEQ |
In Progress |
|
|
Sphagnum capillifolium |
|
Moss |
|
Qiu planned |
|
|
39 |
Takakia ceratophylla |
|
Takakia |
Tissue Prep |
Tissue Prep |
|
|
39.5 |
Tetraphis pellucida |
|
Moss |
RCA |
RCA/CLONE |
|
|
40 |
Physcomitrella patens |
|
Moss |
AP005672 |
Sato |
|
|
41 |
Andreaea sp. |
|
Moss |
Tissue Prep |
Tissue Prep |
|
|
42 |
Tortula ruralis |
|
Moss |
ASSEMBLY |
ASSEMBLY |
|
Assembly is nearing completion. Finishing will be handed to Mel Oliver |
43 |
Selaginella moellendorffi |
|
Lycopod |
SEQ |
RCA/CLONE |
|
Nuclear genome being sequenced at JGI |
|
Selaginella uncinata |
|
Lycopod |
Ueda complete |
|
|
|
44 |
Isoetes flaccida |
|
Lycopod |
SEQ |
SEQ |
|
Knoop working on I. engelmanii mitochondrial genome |
45 |
Huperzia lucidula |
|
Lycopod |
AY660566 |
BAC |
|
Dec 2004: chloroplast genome annotation complete. Manuscript accepted
by Gene |
46 |
Equisetum arvense |
|
Horsetail |
SEQ |
SEQ |
|
|
|
Equisetum hyemale |
|
Horsetail |
|
Qiu planned |
|
|
47 |
Psilotum nudum |
|
Psilotaceae |
AP004638
SEQ |
SEQ |
|
|
48 |
Ophioglossum sp. |
Botrychium virginianum |
Eusporang. Fern |
RCA |
RCA |
In Progress |
|
49 |
Angiopteris evecta |
|
Basal monilophyte |
ASSEMBLY |
CLONE |
|
September 2004: Finishing in Wolf lab |
50 |
Osmunda cinnamomea |
O. regalis var. spectabilis |
Basal fern |
Tissue Prep |
Tissue Prep |
|
|
|
Adiantum capillus-veneris |
|
"Modern" fern |
AY178864 |
|
|
|
51 |
Ceratopteris richardii |
|
"Modern" fern |
QC |
QC |
|
|
52 |
Marsilea quadrifolia |
M. minuta |
Heterosp. Fern |
Tissue prep |
Tissue prep |
|
|
Key for sequencing status, order of stages:
for FACS approach: Tissue prep (processing tissue) → FACS (fluorescence
activated cell sorting) → RCA (rolling-circle amplification) →
CLONE (shotgun)→ SEQ (sequencing) → ASSEMBLY → ANNOTATION.
BAC Library Pipeline: PREP (Culture/Tissue Preparation) → Plugs
→ Clone → Array → QC (5-step QC) → Complete
OGMP = Organelle
Genome Megasequencing Program |